CRYOPRESERVATION OF PRIMARY SPLENOCYTES FROM ANTIBODY PRODUCED MICE AGAINST Bitis arientans SNAKE VENOM

Bitis Arientans are associated with the highest number of morbidity and mortality in Nigeria.The most effective treatments against snakebite is administration antivenom.Antibodies that specific for a single epitope an antigen obtain by isolating antibody-secreting lymphocytes. These lymphocytes found within Splenocytes cells which can be used wide variety immunology-based applications. However, development hybridoma cell rare time consuming. Therefore, valuable materials saved through cryopreservation. This research aimed at Cryopreservation primary splenocytes from Antibody produced mice arientans snake venom. The LD50 venom toxicity was determined according to World Health Organization guidelines. production achieved using six after immunization weeks antibody titer were indirect ELISA method. Myeloma line X63 Ag8.653 cultured Mouse immune response removed aseptically mechanical method viability then isolated cryopreserved cryovial tube stored -80C.The 1.8mg/kg Elisa analysis showed 2 6 elicited IgG Concentration 3.1µg/ml 4.6µg/ml. counted 6.5x10 1.2x10 myeloma 1.95x105 cells.In this finding mouse cryopreserved. Thus, generation monoclonal